Characterization of sterol carrier protein binding with 7-dehydrocholesterol and vitamin D3.
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چکیده
منابع مشابه
Binding of 7-dehydrocholesterol to sterol carrier protein and vitamin D3 effect.
It was confirmed that delta 5,7-sterol delta 7-reductase activity was suppressed by cholecalciferol (vitamin D3) in the enzyme system consisted of microsomes and sterol carrier protein (SCP). The enzyme activity was significantly decreased in the combination with microsomes obtained from either vitamin D-deficient or vitamin D3-treated rat liver and with SCP obtained from vitamin D3-treated rat...
متن کاملSpecific binding protein for active metabolites of vitamin D3.
FIG. 1 Separation of the specific protein binding with vitamin D3 and its metabolites in rat intes tinal cytoplasm by gel filtration on Sephadex G 200 after the administration of vitamin D3-3H in vivo. 1,000I.U. (3•~105cpm) of randomly labeled vitamin D3 (sp. act. 1.2•~104cpm/ug) was injected intracar dially to vitamin D deficient rat 16 hours before decapitation. 2.0 ml of rat intestinal cytop...
متن کاملSterol metabolism. 6. The interconversion of cholesterol, 7-dehydrocholesterol and lathosterol in the rat.
The three closely related sterols, cholesterol, 7-dehydrocholesterol and lathosterol, occur in animal tissues in different proportions. From their structures they would appear to be interconvertible by simple dehydrogenation or reduction steps as shown in Fig. 1, but unequivocal evidence for such a mechanism has not yet been obtained. It has generally been assumed that 7-dehydrocholesterol (pro...
متن کاملA yeast sterol carrier protein with fatty-acid and fatty-acyl-CoA binding activity.
The 14-kDa sterol carrier protein 2 (SCP2) domain is present in Eukaria, Bacteria and Archaea, and has been implicated in the transport and metabolism of lipids. We report the cloning, expression, purification and physicochemical characterization of a SCP2 from the yeast Yarrowia lipolytica (YLSCP2). Analytical size-exclusion chromatography, circular dichroism and fluorescence spectra, indicate...
متن کاملFibroblast membrane sterol kinetic domains: modulation by sterol carrier protein-2 and liver fatty acid binding protein.
The mechanism(s) of intracellular sterol trafficking among subcellular organelle membranes is not well understood. Relative contributions of vesicular, sterol carrier protein, and membrane sterol domain pathways are not resolved. A sterol kinetic assay was used to resolve multiple sterol domains in microsome (MICRO), mitochondria (MITO), and plasma (PM) membrane: exchangeable, 20-40% of total; ...
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ژورنال
عنوان ژورنال: Journal of Nutritional Science and Vitaminology
سال: 1977
ISSN: 0301-4800,1881-7742
DOI: 10.3177/jnsv.23.53